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. 2022 Mar 3;23(5):e54117. doi: 10.15252/embr.202154117

Figure 2. CircHIPK3 sponges miR‐29b and miR‐30c.

Figure 2

  • A, B
    qPCR analysis of TREx 0 and 24 post‐induction (n = 3) using GAPDH as a housekeeper, analysis is of levels of circHIPK3 (A) and HIPK3 (B).
  • C
    FISH analysis of TREx cells reactivated for 16 h with probes against circHIPK3 (red). DAPI was used as nuclei stain (blue), scale bars represent 5 μm on upper panel and 10 μm on lower panel. The lower panel shows a wider field of view.
  • D
    qPCR analysis of Ago2 RIPs in TREx cells transfected with scr, miR‐29b or miR‐30c showing circHIPK3 enrichment over GAPDH, with each repeat normalised to an IgG scr RIP (n = 3).
  • E
    qPCR analysis of Streptavidin RIPs of circHIPK3 or GAPDH enrichment in HEK‐293T cells transfected with either biotinylated miR‐29b or miR‐30c over scr (n = 4).

Data information: In (A, B, D and E) data are presented as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001 (unpaired Student’s t‐test). All repeats are biological.

Source data are available online for this figure.