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. 2022 Apr 24;18(7):3082–3101. doi: 10.7150/ijbs.70524

Figure 6.

Figure 6

STAT3 activation downregulates miR-193a-3p expression. (A) AGS cells transfected with siSTAT3 had a high miR-193a-3p expression determined by RT-qPCR. (B) The PGL4 plasmids carrying different truncations of miR-193a-3p promoter and PCDNA3.1 vector containing STAT3 were transfected in 293T cells, and the relative luciferase activity was measured after 24h. (C) ChIP assay demonstrated the direct binding of STAT3 to the miR-193a-3p promoter. (D) Operation flow diagram of single-molecular magnetic tweezers. (E-F) Force vs. time and DNA extension vs. time curve for promoter of miR-193a-3p with or without p-STAT3. Error bars, SD. *P < 0.05; **P < 0.01; ***P < 0.001.