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. 2022 Apr 24;18(7):3082–3101. doi: 10.7150/ijbs.70524

Figure 7.

Figure 7

The miR-193a-3p inhibits LAMC1 expression in a post-transcriptional manner. (A-B) AGS cells were transfected with miR-193a-3p mimic, inhibitor or NC, and agarose gel electrophoresis and RT-qPCR were used to measure RNA level change, Western blots and ELISA for protein level. (C) The AGS cells were transfected with siLAMC1, RT-qPCR for miR-193a-3p expression. (D) A structure diagram of the pmirGLO dual-luciferase reporter vector with 3′-UTR of LAMC1 mRNA harbors miR-193a-3p binding sites. (E) The relative luciferase activity of reporter plasmids carrying wild-type or mutant LAMC1 3′-UTR. (F) The LAMC1 mRNA decay curves of AGS cells carrying miR-193a-3p mimic or mimic NC. (G) The AGS cells were transfected with miR-193a-3p mimic or mimic NC for 48h, and AGO2-RNA immunoprecipitation assay (RIP) was used for analyzed the combination method of miR-193a-3p and LAMC1. Error bars, SD. *P < 0.05; **P < 0.01; ***P < 0.001.