Figure 3.

(C) Western blot analysis for nuclear translocation of p65 subunit of NF-κB in LPS-stimulated (30 min) CT26.WT cells. MD2 blockade was made by L6H21 pretreatment (10 μM for 1 h), pretreatment with MD2 neutralizing antibody (anti-MD2; 1 μg/ml for 1 h), or transfection with siRNA target sequences (siMD2) or negative control sequences (siNC). Cytosolic and nuclear protein was extracted for Western blot analysis. Representative images and quantification of the immune-reactive bands were shown, n=3. Data are shown as mean ± SEM; **, P<0.01, ***, P<0.001, compared to LPS alone.