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. 2022 Apr 18;18(7):2949–2961. doi: 10.7150/ijbs.68438

Figure 3.

Figure 3

The regulatory effect of UCP1 on the metastasis and proliferation process of breast cancer may through mitophagy and pyroptosis activation. (A) Confocal images of UCP1 (green fluorescence) and mitochondrial (red fluorescence) in MDA-MB-231 with UCP1 overexpressed. (B) Electron microscope image of MDA-MB-231 with vector or UCP1. (C) TMRM stain to detect the membrane potential of MDA-MB-231 and (D) BT549 with vector or UCP1 overexpressed. (E) Ros level in MDA-MB-231 with vector or UCP1. (F) Protein expression of PINK-1 and parkin in MDA-MB-231 and BT549 with vector or UCP1 overexpressed. (G) Protein expression of mitochondrial apoptosis factors in MDA-MB-231 and BT549 with vector or UCP1 overexpressed. (H) Protein expression of pro-caspase-3 and caspase-3 in MDA-MB-231 and (I) in BT549 with vector or UCP1 overexpressed. (J) Protein expression and Immunohistochemical staining (K) of GSDME in MDA-MB-231 and BT549 with vector or UCP1 overexpressed.