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. Author manuscript; available in PMC: 2022 May 3.
Published in final edited form as: Oncogene. 2022 Jan 20;41(8):1129–1139. doi: 10.1038/s41388-021-02154-0

Figure 5. Inhibition of PDH phosphorylation reduces cell survival of BAP1 mutant UM cells.

Figure 5.

a. Efficiency of DCA in UM cells was determined by detecting downregulation of p-PDH after 5mM DCA treatment for 48 hours. b. BAP1 wild type (92.1 and MM66) and mutant (MP46, MP65, MP38 and MM28) cells were treated with DCA (5, 7.5 or 10 mM) for 3 days. Changes of cell viability were measured by crystal violet staining. Quantification bar graph represents relative fold change of cell viability with DCA treatments in cells. Representative images of crystal violet are shown. Scale bar: 100 μm. Data are shown as mean ± SEM from biological replicate experiments (n=4). c. Effect of siPDHK1 on MP65 cell growth was analyzed through Incucyte Live Cell Analysis Imaging System. Data are shown as mean ± SEM from biological replicate experiments. *p<0.05, **<0.01, ***<0.001, ****<0.0001 and not significant (ns).