Fig. 6. Knockdown of CRNDE in xenograft tumor in vivo. Cal27 cells (1.0 × 10⁷) stably transfected with blank control, sh-CRNDE or sh-NC were subcutaneously implanted into nude mice (n = 10). On day 35 after cancer cell implantation, all mice were killed. (A) Tumor volume was measured by a calliper every week after implantation for 7 days. (B) Tumor weight was calculated. (C) CRNDE expression was detected by qRT-PCR in xenograft tissues. (D) and (E) The expression levels of PI3K, mTOR, AKT, p-PI3K, p-mTOR and p-AKT were analyzed by Western blot in excised tumor tissues. *P < 0.05 or **P < 0.01 or ***P < 0.001.