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. 2022 Apr 24;13(7):2388–2396. doi: 10.7150/jca.71526

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Wnt5a enhances autophagy. HNSCC CNE2 and 6‐10B cells were infected with lentivirus mediated Wnt5a or control cDNA and then subjected to puromycin screening for 2 weeks. (A) Representative images of autophagosomes (indicated by red arrows) observed by transmission electron microscopy in CNE2 and 6‐10B cells at 3 hours post 4 Gy irradiation exposure. (B) Western blotting was used to examine the expression of proteins associated with the Wnt5a and autophagic proteins (Beclin1 and LC3B). (C) The Pearson rank correlation coefficient validated the positive correlation between Wnt5a and Beclin1.