Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Steady-state kinetic parameters of highly purified CfxA2 β-lactamase cloned in E. coli NI-142
| Substrate | Relative Vmaxa | Km (μM) | Relative Vmax/Km |
|---|---|---|---|
| Benzylpenicillin | 100 | 20.7 | 4.8 |
| Ampicillin | 160 | 38.0 | 4.2 |
| Cefazolin | 300 | 12.3 | 24.4 |
| Cefuroxime | 1,500 | 60.6 | 24.8 |
| Cefotaxime | 600 | 12.9 | 46.5 |
| Cephalothin | 40 | 24.7 | 1.6 |
| Cefoxitin | <0.01 |
Values relative to that of benzylpenicillin, which was set at 100.
