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Comparison of kinetic parameters of partially purified CfxA and CfxA2 β-lactamases obtained from wild-type strains (CfxA, B. vulgatus CLA-341; CfxA2, B. vulgatus NI-2869)
| Substrate | Relative Vmaxa
|
Km (μM)
|
||
|---|---|---|---|---|
| CfxA | CfxA2 | CfxA | CfxA2 | |
| Benzylpenicillin | 100 | 100 | 30.0 | 20.0 |
| Ampicillin | 35 | 130 | 40.0 | 40.0 |
| Cefazolin | 740 | 170 | 133.3 | 10.2 |
| Cefotaxime | 90 | 670 | 40.0 | 8.0 |
| Cefoxitin | <0.01 | <0.01 | ||
Values relative to that of benzylpenicillin, which was set at 100.
