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. 2022 Mar 28;13(7):2040–2049. doi: 10.7150/jca.65167

Figure 5.

Figure 5

miR-1247-3p directly binds to STAT5A mRNA 3' UTR. A. Plasmids, both mutant type and wild-type, of STAT5A mRNA 3' UTR were constructed on the active binding sites of miR-1247-3p and mRNA of STAT5A gene. B-C. The binding of miR-1247-3p with wild type plasmid and not the mutant type was verified by the dual luciferase assay. D. Sequence of siRNA of STAT5A exhibiting the best interference effect was identified and separated for further experiments. E. Higher survival rate of inhibitor group as compared to LV-INC group upon treatment with Docetaxel. No statistical significance between LV-INC+STAT5A siRNA and LV-inhibitor+STAT5A siRNA group was observed. F. Higher survival rate of inhibitor group as compared to the LV-INC group upon treatment with Doxorubicin. No statistical difference between LV-INC+STAT5A siRNA and LV-inhibitor+STAT5A siRNA groups was observed. G. Higher survival rate of inhibitor group as compared to the LV-INC group upon treatment with Gefitinib. No statistical difference between LV-INC+STAT5A siRNA and LV-inhibitor+STAT5A siRNA groups was observed. ***p<0.001 compared with group NC, LV-INC, or LV-mNC. Lentiviruses-inhibitor normal control (LV-INC), Lentiviruses-inhibitor (LV-inhibitor), Lentiviruses-mimics normal control (LV-mNC), Lentiviruses- mimics (LV-mimics). The experiments were repeated at least 3 independent times.