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. 2022 Apr 18;13(7):2293–2300. doi: 10.7150/jca.69269

Figure 3.

Figure 3

PDZRN4 was decreased and lost the ability of reducing proliferation in androgen-dependent cells (LNCap) upon androgen deprivation. A. qRT-PCR assays of the PDZRN4 mRNA expression of PC cell lines LNCap, DU145, C4-2, and PC-3. B. qRT-PCR assays of the PDZRN4 mRNA expression of LNCap cell treated by charcoal media for gradient days or by normal media (Control). C. Western blotting showing the PDZRN4 levels of LNCap cell treated by charcoal media for gradient days or by normal media (Control). D. qRT-PCR assays of the PDZRN4 mRNA expression of DU145 and C4-2 cell treated by charcoal media for gradient days or by normal media (Control). E. Western blotting showing PDZRN4 levels of DU145 and C4-2 cell treated by charcoal media for gradient days or by normal media (Control). F, G. MTT assays displaying the proliferation of LNCap and DU145 cell transfected by PDZRN4 shRNA lentivirus (PDZRN4-kd) or control (Con) cultured by charcoal media.