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The conditions for the TaqMan™ SARS-CoV-2 Mutation Panel reagent (Applied Biosystems) on the Applied Biosystem 7500 Equipment.
| Step | Temperature | Time | Number of Cycles |
|---|---|---|---|
| Reverse Transcription[1] | 50 °C | 10 s | 1 |
| Fast DNA polymerase activation[2] | 90 °C | 2 s | 1 |
| Denaturation | 90 °C | 3 s | 45 |
| Anneal / extend | 60 °C | 30 s | |
| Post-read | 60 °C | 30 s | 1 |
