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. 2022 Feb 22;10(2):e003663. doi: 10.1136/jitc-2021-003663

Figure 2.

Figure 2

Loss of YTHDF1 in GC cell lines suppresses growth in vitro and tumor growth in immunodeficient mice. (A) Knockdown or knockout of YTHDF1 protein in AGS, BGC823, MKN74, and YTN16 cell lines were confirmed by western blot. (B) Cell viability was determined in GC cell lines with YTHDF1 knockdown or knockout by MTT assay or cell counting. (C) Colony formation assay of GC cells with YTHDF1 knockdown or knockout. (D) MKN74 cells (5×106 cells per tumor) expressing control vector or YTHDF1-shRNA were injected into the right dorsal flanks of NSG mice (n=6 for each group). Mice were sacrificed 7 weeks after injection. Representative tumor images, tumor volume, and tumor weight were shown. (E) YTN16 cells (5×106 cells per tumor) with control vector or knockout of YTHDF1 were injected into the right dorsal flanks of NSG mice (n=6 for each group). Mice were sacrificed 6 weeks after injection. Representative tumor images, tumor volume, and tumor weight were shown. *P<0.05; **p<0.01; ***p<0.001. GC, gastric cancer; NSG, NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ.