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. 2022 Feb 22;10(2):e003978. doi: 10.1136/jitc-2021-003978

Figure 1.

Figure 1

Binding of mutated peptides to HLA-A*02.01 molecules. (A) Sequences of mutated peptides predicted as binders to HLA-A*02.01 molecules with their corresponding WT version. (B) Mutated peptides were tested in binding assays at 100 µM using T2 cells. Results are expressed as Fluorescence Index (FI). Peptides above the 0.5 FI threshold (horizontal bar) were considered positive. Results correspond to the mean of three to six different assays. (C) Selected binder peptides were titrated with decreasing peptide concentrations.