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. 2022 Feb 22;10(2):e003978. doi: 10.1136/jitc-2021-003978

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© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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Identification of a new set of immunogenic neoAgs. (A) Sequences of mutated peptides with changes at non-anchor positions and predicted as binders to HLA-A*02.01 or HLA-DRB1*01 molecules, with their corresponding WT version. (B) Mutated peptides with predicted binding capacity to HLA-A*02.01 were tested in binding assays with T2 cells. Results are expressed as Fluorescence Index (FI) and peptides with FI >0.5 were considered positive. (C) HHD-DR1 mice (n=3 mice/peptide) were immunized with mutated peptides with changes at non-anchor positions and responses were evaluated by Elispot. (D) Mutated peptides with predicted binding capacity to HLA-DRB1*01 were tested in binding assays with HOM2 cells. Results are expressed as Binding Score (BS) and peptides with BS >0.5 were considered positive. (E) Immunogenic peptides (n=3 mice/peptide) were also tested against decreasing concentrations of mutated and WT peptides.