Figure 3.

The need for tryptophan and the blockage of chitin synthesis of the chs5∆ mutant are differentially suppressed by several mutations affecting TGN traffic. (A) Growth of the indicated strains in YEPD media with the indicated supplements. (B) Growth of the indicated strains in SD w/o Trp media supplemented with the indicated concentrations of Trp. (C, D) Growth of the indicated strains in YEPD media with the indicated supplements. All strains used are W303 derivatives grown O/N in YEPD media, serially diluted and plated onto the indicated media. Each panel represents a single experiment using a unique set of plates; note the slight differences in the growth of similar strains between different panels/experiments. (E) Calcofluor staining of the indicated strains. Cells grown in YEPD media were stained for 1.5 hours with calcofluor (50 μg/ml). Note the reduction in calcofluor staining shown by all chs5∆ strains that is partially reverted by gga2∆, gga1∆/gga2∆ and aps1∆ concomitant mutations. This result coincides with their higher level of sensitivity to this drug, which is shown in the other figure panels.