Table 1.
Yeast strains used.
| Strain | Genotype | Origin / Reference |
|---|---|---|
| S. cerevisiae strains | ||
| CRM67 | W303, mat a, (leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15) | Lab. collection |
| CRM2268 | W303, mat a, chs5Δ::natMx4 | Lab. Collection |
| CRM3066 | W303, mat a, bch1 Δ::kanMx4 bud7Δ::natMx4 | (42) |
| CRM3081 | W303, mat a, chs6Δ::kanMx4 bch2Δ::natMx4 | (42) |
| CRM3851 | W303, mat a, tat2Δ::hphNT1 | This study |
| CRM3853 | W303, mat a, chs5Δ::natMx4 tat2Δ::hphNT1 | This study |
| CRM3909 | W303, mat a, PGAL1-TAT2::KanMx4 | This study |
| CRM3917 | W303, mat a, chs5Δ::natMx4 PGAL1-TAT2::KanMx4 | This study |
| CRM2868 | W303, mat a, PGAL1-GFP-SSY1::KanMx4 | This study |
| CRM2871 | W303, mat a, chs5Δ::natMx4 PGAL1-GFP-SSY1 ::KanMx4 | This study |
| CRM3811 | W303, mat a, STP1-3xHA::hphNT1 | This study |
| CRM3825 | W303, mat a, STP1-3xHA::hphNT1 chs5Δ::natMx4 | This study |
| CRM3813 | W303, mat a, GLN3-3xHa::hphNT1 | This study |
| CRM3827 | W303, mat a, GLN3-3XHΔ::hphNT1 chs5Δ::natMx4 | This study |
| CRM3023 | W303, mat a, GTR1-GFP::hphNT1 | This study |
| CRM3032 | W303, mat a, chs5Δ::natMx4 GTR1-GFP::hphNT1 | This study |
| CRM3017 | W303, mat a, TCO89-GFP::hphNT1 | This study |
| CRM3020 | W303, mat a, chs5Δ::natMx4 TCO89-GFP::hphNT1 | This study |
| CRM2972 | W303, mat a, GAP1-GFP::hphNT1 | This study |
| CRM2979 | W303, mat a, chs5Δ::natMx4 GAP1-GFP::hphNT1 | This study |
| CRM2894 | W303, mat a, TAT2-GFP::hphNT1 | This study |
| CRM2903 | W303, mat a, chs5Δ::natMx4 TAT2-GFP::hphNT1 | This study |
| CRM3531 | W303, mat a, MUP1-GFP::KanMx4 | This study |
| CRM3540 | W303, mat a, MUP1-GFP::KanMx4 chs5Δ::natMx4 | This study |
| CRM3882 | W303, mat a, TAT252-53-3xHA (internal by Delitto Perfetto) | This study |
| CRM3890 | W303, mat a, TAT252-53-3xHA chs5Δ:: kanMx4 | This study |
| CRM3862 | W303, mat a, bul1Δ:: kanMx4 | This study |
| CRM3903 | W303, mat a, bul2Δ:: hphNT1 | This study |
| CRM3880 | W303, mat a, bul1Δ:: kanMx4 bul2Δ:: hphNTl | This study |
| CRM3864 | W303, mat a, chs5Δ:: kanMx4 bul1Δ:: kanMx4 | This study |
| CRM3950 | W303 mat a, gga2Δ:: hphNT1 chs5Δ::kanMx4 | This study |
| CRM3526 | W303 mat a, rcy1Δ:: kanMx4 gga1A:: natMx4 | Lab. Collection |
| CRM3602 | W303 mat a, rcy1Δ:: kanMx4 gga2Δ:: hphNT1 | Lab. Collection |
| CRM4025 | W303 mat a, rcy1Δ:: kanMx4 aps1Δ:: hphNT1 | This study |
| CRM3432 | W303 mat a, APL4-VC::kanMx4 CHS5-VN::HIS3 | This study |
| CRM3477 | W303 mat a, CHS5-VC::kanMx4 | This study |
| CRM3997 | W303 mat a, CHS5-VN::kanMx4 | This study |
| CRM4003 | W303 mat a, CHS5-VN::kanMx4 MUP1-VC::HIS3 | This study |
| CRM4070 | W303 mat a, CHS5-VN::kanMx4 GGA2-VC::HIS3 | This study |
| CRM2879 | W303 mat a, SEC7-mRuby2::kanMx4 | This study |
| CRM2882 | W303 mat a, chs5Δ::natMx4 SEC7-mRuby2::kanMx4 | This study |
| CRM4088 | W303 mat a, arf1Δ::kanMx4 | This study |
| CRM4090 | W303 mat a, chs5Δ::natMx4 arf1Δ::kanMx4 | This study |
| CRM1278 | W303 mat a, chs3A:: URA3 chs5Δ:: natMx4 | Lab. Collection |
| CRM1590 | W303 mat a, chs3A:: natMx4 | Lab. Collection |
| CRM3089 | W303, mat a, bch1Δ::kanMx4 bud7Δ:: natMx4 chs3Δ::hphNT1 | This study |
| CRM3091 | W303 mat a, chs6Δ::kanMx4 bch2Δ:: natMx4 chs3Δ::hphNT1 | This study |
| CRM4098 | W303 mat a, chs3Δ:: URA3 chs6Δ::kanMx4 | This study |
| CRM3534 | W303 mat a, CHS5-VC::kanMx4 gga2Δ:: hphNT1 | This study |
| CRM3511 | W303 mat a, CHS5-VC::kanMx4 chs6Δ:: natMx4 bch2A::hphNT1 | This study |
| CRM3668 | W303 mat a, CHS5-VC::kanMx4 bch1Δ:: hphNT1 bud7A:: natMx4 | This study |
| CRM3641 | W303 mat a, CHS5-VC::kanMx4 chs7Δ:: hphNT1 | This study |
| CRM3674 | W303 mat a, CHS5-VC::kanMx4 chs3Δ:: URA3 | This study |
| CRM3511 | W303 mat a, CHS5-VC::kanMx4 chs6Δ:: natMx4 | This study |
| CRM3676 | W303 mat a, CHS5-VC::kanMx4 chs6Δ:: natMx4 chs3Δ:: URA3 | This study |
| CRM3432 | W303 mat a, APL4-VC::kanMx4 | This study |
| CRM3453 | W303 mat a, APL4-VC::kanMx4 gga2Δ:: hphNT1 | This study |
| CRM3436 | W303 mat a, chs3Δ::URA3 APL4-VC::kanMx4 | This study |
| CRM2248 | W303, mat a, CHS5-mCherry::natMx4 APS1-GFP:: hphNT1 | Lab. Collection |
| CRM2533 | W303, mat a, CHS5-mCherry::natMx4 GGA1-GFP:: hphNT1 | Lab. Collection |
| CRM808 | BY4741, mat a (his3A1, leu2A0, met15A0, ura3A0) | EUROSCARF |
| CRM1435 | BY4741, mat a, chs3Δ::natMx4 | Lab. Collection |
| CRM2453 | BY4741, mat a, chs3Δ::natMx4 chs5Δ::hphNT1 | Lab. Collection |
| CRM3922 | BY4741, mat a, chs5Δ::natMx4 | Lab. Collection |
| CRM3924 | BY4741, mat a, art1Δ::kanMx4chs5Δ::natMx4 | This study |
| CRM3926 | BY4741, mat a, art2Δ::kanMx4chs5Δ::natMx4 | This study |
| CRM3928 | BY4741, mat a, art3Δ::kanMx4chs5Δ::natMx4 | This study |
| CRM3930 | BY4741, mat a, art4Δ::kanMx4chs5Δ::natMx4 | This study |
| CRM3932 | BY4741, mat a, art5Δ::kanMx4chs5Δ::natMx4 | This study |
| CRM3934 | BY4741, mat a, art6Δ::kanMx4chs5Δ::natMx4 | This study |
| CRM3935 | BY4741, mat a, art7Δ::kanMx4chs5Δ::natMx4 | This study |
| CRM3937 | BY4741, mat a, art8Δ::kanMx4chs5Δ::natMx4 | This study |
| CRM3939 | BY4741, mat a, art9Δ::kanMx4chs5Δ::natMx4 | This study |
| CRM3941 | BY4741, mat a, art10Δ::kanMx4 chs5Δ::natMx4 | This study |
| CRM4019 | BY4741, mat a, GGA2-GFP::hphNT1 APL4-mCherry:: natMx4 | This study |
| CRM2761 | X2180-1A, mat a (SUC2 mal mel gal2 CUP1) | Francisco del Rey |
| CRM2763 | X2180-1A, mat a, chs5Δ::kanMx4 | This study |
| CRM3957 | X2180-1A, mat a, tat2Δ::kanMx4 | This study |
| CRM3959 | X2180-1A, mat a, chs5Δ::kanMx4 tat2Δ::kanMx4 | This study |
| CRM2783 | X2180-1A, mat a, ssy1Δ::natMx4 | This study |
| CRM3010 | X2180-1A, mat a, ssy1Δ::natMx4 chs5Δ::kanMx4 | This study |
| CRM4311 | W303, mat a, ARF1-GFP: :hphNT1 | This study |
| CRM4134 | W303, mat a, CHS3-2xGFP::hphNT1 | This study |
| CRM4136 | W303, mat a, CHS3-2xGFP::hphNT1 chs5Δ::natMx4 | This study |
| CRM4140 | W303, mat a, CHS3-2xGFP::hphNT1 arf1Δ::kanMx4 | This study |
| CRM4138 | W303, mat a, CHS3-2xGFP::hphNT1 chs5Δ::natMx4 arf1Δ::kanMx4 | This study |
| CRM4157 | W303, mat a, PGAL1-ARF1::KanMx4 | This study |
| CRM4159 | W303, mat a, chs5Δ::natMx4 PGAL1-ARF1::KanMx4 | This study |
| CRM4302 | W303, mat a, PGAL1-SEC7::KanMx4 | This study |
| CRM4339 | W303, mat a, PGAL1-SEC7::KanMx4 chs5Δ::natMx4 | This study |
| CRM2818 | BY4741, mat a, CHS5-GFP::hphNT1 | Lab. Collection |
| CRM4160 | BY4741, mat a, CHS5-GFP::hphNT1 arf1Δ::kanMx4 | This study |
| CRM4594 | W303, mat a, CHS5-VN::kanMx4 ANP1-VC::HIS3 | This study |
| CRM4586 | W303, mat a, CHS5-mCherry::natMx4 GGA1-GFP::hphNT1 arf1Δ:: kanMx4 | This study |
| CRM4584 | W303, mat a, CHS5-GFP::hphNT1 | This study |
| CRM4573 | W303, mat a, PGAL1-GGA2::KanMx4 CHS5-GFP::hphNT1 | This study |
| CRM4118 | W303, mat a, APS1-GFP::hphNT1 | This study |
| CRM4153 | W303, mat a, PGAL1-GGA2::KanMx4 APS1-GFP::hphNT1 | This study |
| CRM4571 | W303, mat a, PGAL1-BUD7::KanMx4 APS1-GFP::hphNT1 | This study |
| CRM4576 | W303, mat a, PGAL1-BCH1::KanMx4 APS1-GFP::hphNT1 | This study |
| C. albicans strains | ||
| CRM2499 | BWP17, (ura3::imm434/ura3::imm434 his1 ::hisG/his1::hisG arg4: :hisG/arg4::hisG) | (75) |
| CRM2531 | BWP17, Cachs5Δ::ARG4/Cachs5Δ::HIS1 | Lab. Collection |
| CRM3258 | BWP17, Caaps1Δ::SAT1/Caaps1 Δ::URA3 | This study |
| CRM3261 | BWP17, Cachs5Δ::ARG4/Cachs5Δ::HIS1 Caaps1Δ::SAT1/Caaps1Δ::URA3 | This study |