Fig. 4.

Influence of mucin-producing LS174T cells on growth and adherence of EPEC, L. reuteri and R. gnavus. Bacterial strains were incubated in the VDC with T84 (−) or T84/LS174T (+) epithelia for 4 h. (A) Growth of planktonic bacteria and epithelial adherence were evaluated by quantifying CFU in apical media and cell lysates, respectively. Shown are individual data points. Means are indicated by a line. Data were analysed using unpaired t-test, *P<0.05, **P<0.01. (B) Binding of EPEC and R. gnavus (RG) to T84/LS174T epithelia. Bacteria are labelled in red and LS174T cells are stained with anti-MUC2 (green). Scale bars: 10 µm.