Fig. 8.

Effect of L. reuteri and R. gnavus on IL-8 release during EPEC infection. T84/LS174T epithelia were incubated with EPEC, L. reuteri (LR), EPEC and L. reuteri (EPEC LR) or left non-infected (NI) (left). In addition, incubations were performed with EPEC, R. gnavus (RG), EPEC and R. gnavus (EPEC RG) and NI controls (right). After 4 h, media in apical chambers were exchanged for media containing 50 µg/ml gentamicin (GEN, top) or 16 µg/ml polymyxin B (PMB, bottom), and cultures were continued for 18 h. IL-8 levels in apical supernatants were determined by ELISA. Data are shown as individual data points with means indicated by a line. Statistical analysis was performed by one-way ANOVA with Tukey's multiple comparison test. Groups with different letters are significantly different.