Figure 1.

FFPE transcriptome classification of ACT in the training cohort. (A) Unsupervised classification of ACT based on the 2000 most variable genes identifies three main groups, corresponding to ‘C1A’ aggressive carcinomas, ‘C1B’ indolent carcinomas and ‘C2’ adenomas. (B) Heatmap of the three non-negative matrix factorization (NMF) ranks used for generating the unsupervised classification. (C) Expression of steroid (adrenal differentiation), proliferation and immune signatures in each ACT. (D) Expression profiles of the top 50 most significantly different genes among the three transcriptome groups. (E) Consensus matrix representing the similarity between samples over iterative clustering algorithms. Rows and columns are patient samples. Consensus matrix values range from 0 (never clustered together) to 1 (always clustered together).