Figure 3.

circPTPRM interacts with miR-139-5p in vitro. (a) Potential interaction between circPTPRM and miR-139-5p was identified by the bioinformatic analysis using Starbase v2.0 (http://starbase.sysu.edu.cn/index.php). (b) Luciferase activities of PTPRM (PTPRM-WT) and PTPRM with the miRNA-binding site mutant (PTPRM-MUT) were determined by luciferase reporter gene assays in the A549 cells treated with NC mimics, miR-139-5p mimic, respectively. (c) The interaction of PTPRM with wild-type (WT) miR-139-5p (bio-miR-139-5p), miR-139-5p with the PTPRM-binding site mutant (bio-miR-139-5p MUT) was examined by RNA pull-down followed by qRT-PCR in the A549 cells. (d) The expression levels of miR-139-5p were measured by qRT-PCR in the A549 cells infected with the lentiviral plasmids carrying PTPRM shRNA (sh-PTPRM) or corresponding control shRNA (sh-NC). Data are presented as mean ± SD.

Statistical significant differences were indicated as *P < .05, **P < .01. Abbreviations: circPTPRM: circ RNA PTPRM; PTPRM-WT, wild-type reporter vectors PTPRM; PTPRM-MU, mutant-type reporter vectors PTPRM; qRT-PCR: quantitative real-time polymerase chain reaction; SD: standard deviation.