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. 2019 Jul 17;9(39):22240–22247. doi: 10.1039/c9ra03690c

Red-green fluorescence ratio of positive controls and target complexes 1a and 1ba.

Complex Ratio (FL2/FL1)
Negative control 5.42
CCCP 0.04
DCA 4.17
Cisplatin 0.54
Carboplatin 2.56
Oxaliplatin 0.94
1a : DCA (1 : 1) 0.59
1b : DCA (1 : 1) 0.80
1a 0.98
1b 0.86
a

HepG-2 cells cultured in the presence of 30 μM of cisplatin, carboplatin, oxaliplatin, complex 1a or 1b for 48 hours at 37 °C (DMF final concentration < 0.4%). Then the cells were harvested by centrifugation and incubated with JC-1 solution for 30 min. After briefly washing, the proportions of red and green fluorescence intensity were immediately detected and analyzed by flow cytometry (Ex = 488 nm; Em = 530 nm).