Red-green fluorescence ratio of positive controls and target complexes 1a and 1ba.
| Complex | Ratio (FL2/FL1) |
|---|---|
| Negative control | 5.42 |
| CCCP | 0.04 |
| DCA | 4.17 |
| Cisplatin | 0.54 |
| Carboplatin | 2.56 |
| Oxaliplatin | 0.94 |
| 1a : DCA (1 : 1) | 0.59 |
| 1b : DCA (1 : 1) | 0.80 |
| 1a | 0.98 |
| 1b | 0.86 |
HepG-2 cells cultured in the presence of 30 μM of cisplatin, carboplatin, oxaliplatin, complex 1a or 1b for 48 hours at 37 °C (DMF final concentration < 0.4%). Then the cells were harvested by centrifugation and incubated with JC-1 solution for 30 min. After briefly washing, the proportions of red and green fluorescence intensity were immediately detected and analyzed by flow cytometry (Ex = 488 nm; Em = 530 nm).