Experimental setup and the obtained responses using PBD^a.

Run no	Variables					Response
	A	B	C	D	E	Y1*	Y1**	Y1***
01	9	67.5	20	1.1	Water	0.0021	0.0521	0.0037
02	11	80	5	2.0	Methanol	0.1040	0.1451	0.1691
03	7	55	35	2.0	Methanol	0.2440	0.3476	0.2880
04	7	55	5	2.0	Methanol	0.0554	0.1378	0.0814
05	7	80	35	0.2	Methanol	0.1966	0.1916	0.1036
06	7	80	35	2.0	Water	0.7346	0.5332	0.9830
07	7	80	5	0.2	Water	0.0391	0.0776	0.0264
08	7	55	5	0.2	Water	0.0058	0.0086	0.0070
09	9	67.5	20	1.1	Methanol	0.0040	0.0043	0.0041
10	11	80	5	2.0	Water	0.1871	0.2408	0.1526
11	11	55	35	0.2	Water	0.0058	0.1148	0.0136
12	9	67.5	20	1.1	Water	0.0045	0.0521	0.0037
13	11	55	35	2.0	Water	0.0890	0.3607	0.1428
14	11	55	5	0.2	Methanol	0.0068	0.0191	0.0043
15	9	67.5	20	1.1	Methanol	0.0063	0.0436	0.0041
16	11	80	35	0.2	Methanol	0.0360	0.1090	0.0569

^a(pH, (A)), (temperature, Temp (B), °C), (reaction time, RT, (C) min), (reagent volume, RV, (D) mL), (solvent type, DS, (E) methanol or distilled water), absorbance (Abs at 469 nm). *Experimental values for Y1. **Predicted values before response transformation: Y1** = −0.176 − 0.0207pH + 0.00360Temp + 0.00699RT + 0.1366volume of NBD-Cl − 0.0479DS − 0.1670Ct Pt. ***Predicted values after response transformation: ln(Y1***) = −7.26 − 0.1499pH + 0.0532Temp + 0.0421RT + 1.307volume of NBD-Cl + 0.051 DS − 2.801Ct Pt.