Analytical parameters for the determination of l-ORN.
Parameter | Value | Parameter | Value |
---|---|---|---|
Wavelength, λmax (nm) | 469 | Slope (b) | 0.0180 |
Linear range, (μg mL−1)a | 5.00–50.00 | Intercept (a) | 0.1036 |
S b | 2.41 × 10−4 | r 2 | 0.9991 |
±tSb | 1.67 × 10−4 | LOD (μg mL−1)b | 1.77 |
S a | 6.90 × 10−3 | LOQ (μg mL−1)b | 5.38 |
±tSa | 4.78 × 10−3 | Residual SS | 4.70 × 10−4 |
S y/x | 9.69 × 10−3 | Regression SS | 0.5257 |
Regression equation: Y = bX + a, where Y is the absorbance, X is concentration in M, a is the intercept, b is the slope, Sb = SD of slope, ±tSb = confidence limit for slope, Sa = SD of intercept ± tSa = confidence limit for intercept Sy/x = SD of the regression, SS is sum of squares.
LOD = limit of detection, LOQ = limit of quantification, and r2 = coefficient of determination.