Fig. 2.

NDR1 is critical for the regulation of cancer stem cell properties in breast cancer cells. a, b NDR1 siRNA or control siRNA were transfected in SUM149 or MCF-7 cells for 72 h. Cells were harvested and subjected to CD24/44 staining and flow cytometry analysis. c, d NDR1 or control vector were expressed in SUM149 or MCF-7 cells for 72 h. Cells were harvested and subjected to CD24/44 staining and flow cytometry analysis. e, f NDR1 siRNA or control siRNA were transfected in SUM149 or MCF-7 cells for 72 h. Cells were harvested and subjected to ALDEFLUOR staining and flow cytometry analysis. g, h NDR1 or control vector were expressed in SUM149 or MCF-7 cells for 72 h. Cells were harvested and subjected to ALDEFLUOR staining and flow cytometry analysis. i, j SUM149 or MCF-7 cells expressed NDR1 shRNA or control vector were subjected to sphere-forming assay. The representative spheres were shown in i. The distribution of sphere diameters was shown in j. k, l SUM149 or MCF-7 cells expressed NDR1 or control vector were subjected to sphere-forming assay. The representative spheres were shown in k. The distribution of sphere diameters was shown in l. The bar represents mean ± SD of three independent experiments (*: p < 0.05, **: p < 0.01, ***: p < 0.001)