Fig. 3.

NDR1 increases Notch1 signaling activity in breast cancer cells. a, b NDR1 or control vector were expressed in SUM149 or MCF-7 cells for 48 h. Cells were harvested and subjected to Real-time PCR analysis. c, d NDR1 siRNA or control siRNA were transfected in SUM149 or MCF-7 cells for 48 h. Cells were harvested and subjected to Real-time PCR analysis. e NDR1 or control vector were expressed in SUM149 or MCF-7 cells for 48 h. Cells were harvested and subjected to western blot analysis. f NDR1 siRNA or control siRNA were transfected in SUM149 or MCF-7 cells for 48 h. Cells were harvested and subjected to western blot analysis. g, h Luciferase reporter (Notch1 or control) and Renilla reporter were transfected into SUM149 or MCF-7 cells for 12 h. Then, NDR1 or control vector was expressed in SUM149 or MCF-7 cells for 48 h. Cells were harvested and subjected to luciferase reporter assay. i, j Luciferase reporter (Notch1 or control) and Renilla reporter were transfected into SUM149 or MCF-7 cells for 12 h. Then, NDR1 siRNA or control siRNA were transfected in SUM149 or MCF-7 cells for 48 h. Cells were harvested and subjected to luciferase reporter assay. The bar represents mean ± SD of three independent experiments (*: p < 0.05, **: p < 0.01, ***: p < 0.001)