Fig. 6.

NDR1 mediates IL-6, TNF-α or Wnt3a induced activation of Notch1 signaling pathway and enrichment of breast cancer stem cells. a–c MCF-7 cells were treated with or without IL-6 or shNDR1 for 7 days. The cell were collected for western blot analysis or CD24/44 analysis. d–f MCF-7 cells were treated with or without TNF-α or shNDR1 for 7 days. The cell were collected for western blot analysis or CD24/44 analysis. g–i MCF-7 cells were treated with or without Wnt3a or shNDR1 for 7 days. The cell were collected for western blot analysis or CD24/44 analysis. The bar represents mean ± SD of three independent experiments (*: p < 0.05, **: p < 0.01, ***: p < 0.001)