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. Author manuscript; available in PMC: 2022 May 3.
Published in final edited form as: J Control Release. 2020 Dec 21;330:329–340. doi: 10.1016/j.jconrel.2020.12.010

Fig. 6.

Fig. 6.

In vitro transfection of ECO/pDNA nanoparticles in ARPE-19 cells in the presence of sucrose or sorbitol. Confocal images (A) and flow cytometry (B, C) of GFP expression in ARPE-19 cells 48 h after transfection using ECO/pCMV–GFP nanoparticles (N/P = 8, DNA concentration of 1 μg/mL, under 0%, 5% and 10% sucrose or sorbitol). (D) ABCA4 mRNA expression 48 h after transfections demonstrated by qRT-PCR using ECO/pCMV–ABCA4 and ECO/pCMV–ABCA4–SV40 nanoparticles (N/P ratios of 6, 8 and 10, DNA concentration of 1 μg/mL, under 0%, 5% and 10% sucrose or sorbitol). ABCA4 mRNA expressions of ECO/pCMV–ABCA4–SV40 nanoparticles were normalized to those of ECO/pCMV–ABCA4 nanoparticles. (Scale bars represent 20 μm. *p < 0.05, relative to the non-treated control in B and C. #p < 0.05, relative to the GFP expression in 5% sorbitol. *p < 0.05, between the two groups under the line in D).