Fig. 4.

Risperidone induces an increase of TNF-α expression and a decrease of SATB2 expression. A relative mRNA expression of TNF-α and SATB2 determined by RT-qPCR in control and Risperidone-treated MC3T3-E1 cells. B–C Western blot analysis of TNF-α and SATB2 protein expression in control and Risperidone-treated MC3T3-E1 cells. The band intensity was assessed. D relative mRNA expression of TNF-α and SATB2 was detected by RT-qPCR in MC3T3-E1 cells. E Western blot analysis of TNF-α and SATB2 protein expression in MC3T3-E1 cells. *p < 0.05, compared with control cells or MC3T3-E1 cells treated with si-NC. ^#p < 0.05, compared with MC3T3-E1 cells treated with Risperidone + si-NC. ^&p < 0.05, compared with MC3T3-E1 cells treated with Risperidone + si-TNF-α. The experiments are repeated 3 times