Fig. 5.

Risperidone inhibits osteoblast differentiation through the TNF-α/SATB2 axis. A the mRNA expression of OPG, collagen I and RANKL in MC3T3-E1 cells determined by RT-qPCR. B Western blot analysis of OPG, collagen I and RANKL expression in MC3T3-E1 cells. The protein band was assessed. C repesentative images and statistical analysis of positive Alizarin red S staining in MC3T3-E1 cells. D repesentative images and statistical analysis of positive ALP staining in MC3T3-E1 cells. *p < 0.05, compared with MC3T3-E1 cells treated with si-NC. ^#p < 0.05, compared with MC3T3-E1 cells treated with Risperidone + si-NC. ^&p < 0.05, compared with MC3T3-E1 cells treated with Risperidone + si-TNF-α. The cell experiments are repeated 3 times