Fig. 6.

Risperidone inhibits autophagy and accelerates apoptosis of osteoblasts via the TNF-α/SATB2 axis. A representative images of autophagosome in MC3T3-E1 cells assessed by immunofluorescence. B Western blot analysis of autophagy-related protein (LC3 II/I, Beclin1, and p62) expression in MC3T3-E1 cells. The band intensity was quantified. C Western blot analysis of apoptosis-related proteins (cleaved PARP1, cleaved caspase-3, cleaved caspase-8 and cleaved caspase-9). The protein band was quantified. D apoptosis rate of MC3T3-E1 cells determined by flow cytometry. *p < 0.05, compared with MC3T3-E1 cells treated with si-NC. ^#p < 0.05, compared with MC3T3-E1 cells treated with Risperidone + si-NC. ^&p < 0.05, compared with MC3T3-E1 cells treated with Risperidone + si-TNF-α. The experiments are repeated 3 times