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. 2022 May 4;20:194. doi: 10.1186/s12967-022-03396-6

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Overexpression of CYP2E1 inhibits the proliferation, migration, and invasion of HCC cells in vitro and in vivo. A CYP2E1 protein expression was evaluated by western blotting in control and CYP2E1-overexpressing HCC cells. B The effect of CYP2E1 overexpression on cellular survival as determined by CCK-8 assays. C Representative images from scratch wound assays of MHCC-97H-CYP2E1 (left panel), SMMC-7721-CYP2E1 (middle panel), and the respective control cells (×100). Statistical graphs of scratch wound assays (right panel). D Representative images and statistical analysis (down panel) of transwell assays for MHCC-97H-CYP2E1, SMMC-7721-CYP2E1, and the respective control cells (×100). E Image and growth curve of xenograft tumors in nude mice injected with the indicated cells (n = 3 in each assay). F Images of the liver orthotopic transplantation tumors in nude mice implanted with the subcutaneous tumors from MHCC-97H-CYP2E1 or MHCC-97H-GFP cells. G Representative H & E images of lung tissue slides of nude mice injected by tail vein with MHCC-97H-CYP2E1 and MHCC-97H-GFP. The data in B–E are presented as the means ± sd. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001 vs. control group