Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 4;20:194. doi: 10.1186/s12967-022-03396-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 3 — CYP2E1 inhibits Wnt/β-catenin signaling via reducing the Dvl2 protein level. A Luciferase activity of TOPFlash in MHCC-97H-CYP2E1, SMMC-7721-CYP2E1 and their respective control cells. Data are shown as mean ± sd (n = 3 in each assay). *P ≤ 0.05, **P ≤ 0.01 vs. control cells. B Protein level of Dvl2, β-catenin, TCF4, LEF1 and c-Myc (Wnt/β-catenin signaling-related molecules) in control and CYP2E1-overexpressing HCC cells measured by western blot. C The protein expression of CYP2E1, Dvl2, β-catenin, and c-Myc in control and CYP2E1-overexpressing HCC cells at 6 h after treatment with the CYP2E1 inhibitor CMZ (40 μM). D The OD450 value of MHCC-97H-CYP2E1, SMMC-7721-CYP2E1 and the respective control cells after treatment with the CYP2E1 inhibitor CMZ (40 μM). E Representative images and statistical graphs (right panel) of transwell assays for the indicated cells after treatment with the CYP2E1 inhibitor CMZ (40 μM) (×100). F Representative images and statistical graphs (right panel) of scratch wound assays of MHCC-97H-CYP2E1, SMMC-7721-CYP2E1 and the respective control cells after treatment with the CYP2E1 inhibitor CMZ (40 μM) (×100). Data in 3D, 3E and 3F are shown as mean ± sd (n = 3–6 in each assay). *P ≤ 0.05, ^**P ≤ 0.01, ***P ≤ 0.001 vs. CYP2E1