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Conversion of H2NP to NP in PBS (A) and in human plasma (B). (A) H2NP in PBS was place on the autosampler at 10 °C in the dark, and then analyzed by HPLC with post-column oxidation method at times indicated. (B) Exogenous H2NP was added to plasma. After deproteinization, samples were analyzed. PBS was added instead of H2NP for the preparation of the plasma alone sample.
