TABLE 3.

Growth rate of E. coli strain TA531 expressing wild-type or mutant rRNA from pKK3535^a

16S rRNA sequence	Doubling time (min) with:
	No aminoglycoside	Kanamycin A at 160 μg/ml
Wild type	57 ± 2	No growth
1406A	60 ± 1	90 ± 6
1408G	57 ± 2	56 ± 1

^aThe doubling time of cells expressing 1408G 16S rRNA in the presence of 160 μg of neomycin B per ml is 57 min. Cells were grown in liquid LB medium containing 100 μg of ampicillin per ml and the indicated aminoglycoside.