TABLE 4.

MICs of various aminoglycosides for E. coli strain TA531 expressing the indicated 16S rRNA^a

Antibiotic	MIC (μg/ml)
	Wild type	1406A	1408G
Kanamycin A	5	640	<1,280
Neomycin	10	5	<1,280
Gentamicin C	5	320	<1,280
Paromomycin	10	5	160
G418	5	640	5
Amikacin	5	1.25	NDb
Apramycin	10	2.5	ND
Tobramycin	2.5	160	ND

^aCells were grown in liquid LB medium containing 200 μg of ampicillin per ml and a twofold dilution (from 1280 to 0.625 μg/ml) of the indicated aminoglycoside. Growth was monitored after 24 h of incubation at 37°C. 

^bND, not determined.