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. 2022 Apr 20;13:882946. doi: 10.3389/fpls.2022.882946

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Copyright © 2022 Ma and Yan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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VIGS of GhSOC1s in G. hirsutum delays flowering time. (A) Albino phenotype of TRV:CLA1 as a control. (B) Delayed flowering phenotype of VIGS plants. Red triangles point the flower buds displayed in the left. CK, empty vector control; VI, silence plants. (C) Detection of the efficiency of gene silencing. The expression was normalized to GhHIS and the maximum expression is set as 100%. Error bars represent the standard deviations of three biological replicates. (D,E) Maturity traits of VIGS plants in branch of first square (D) and days from sowing until square appears (E). Asterisks indicate statistically significant differences among 15 individuals according to Student’s t-test (**P < 0.01). (F) Expression of GhLFY, GhAP1, and GhFT in Silencing and control plants. The expression is normalized to GhHIS and the maximum expression is set as 100%. Error bars represent the standard deviations of three biological replicates.