Fig. 2. Expression and kinase activity of purified recombinant full-length PDK3. (A) Gel image showing the expression of PDK3 with an approximate size of 49.5 kDa. Two serial dilutions of protein were loaded for clarity (lane 1 corresponds to 10 μg of purified protein). (B) ATPase assay of PDK3 showing the kinase activity with increasing amount of ATP.
