Figure 1.

Heterogeneity of MDA-MB-231 human cancer cell migratory capability is heritable and can be sorted based on migration behavior using an in vitro transwell migration assay. a) Fraction of motile MDA-MB-231 cells following seeding in 1.5 mg/mL collagen matrix. Dashed line indicates maximal motile fraction achieved by steady state. b) Single-cell migration paths and c) total and net cell migration speeds over 8 h. d) Correlation of total cell migration speed before and after mitosis. Each pair of daughter cells, D1 and D2, is connected by a vertical line, and the average daughter cell speed was used to determine correlation (R² = 0.62). e) Schematic of migration cell sorting technique. f) Fraction of cells migrated through transwell assay for indicated populations after 4 days. g) Motile fraction of MDA-MB-231 subpopulations and parental cells. h) Single-cell migration paths of MDA-MB-231 subpopulations. i) Migration speeds of MDA-MB-231 subpopulations and MDA-MB-231 parental cells after seeding in 1.5 mg/mL collagen matrix. Data in (a), (f), and (g) display mean ± SEM. Statistical significance in (f) was calculated using one-way ANOVA (n = 3,4,4). Statistical significance in (g) was calculated using one-way ANOVA (n = 10,13,14). In (i), box and whisker plot show medians, 25^th/75^th, and minimum and maximum values. Statistical significance in (i) was calculated using a Kruskal-Wallis H test (n = 34,41,28). * p < 0.05, **** p < 0.0001, n.s., non-significant.