Figure 5.
E-cadherin expression tunes migration ability and mode in phenotypically sorted subpopulations. a) Fraction of cells migrated for MDA subpopulations with and without E-cadherin in transwell assays (n = 3). b) Motile fraction of MDA subpopulations with and without E-cadherin in 1.5mg/mL 3D collagen (n = 10–35). c) Microtrack migration speeds for MDA-MB-231 subpopulations with and without E-cadherin (n = 30–36). d) Representative images of tumor spheroid outgrowth at 48 h post-embedding. Scale bar: 100 μm e) Outgrowth area to quantify cell migration from in vitro tumor spheroids made from MDA-MB-23 subpopulations with and without E-cadherin embedded in 1.5 mg/mL 3D collagen matrix at 48 h. Data from (a) and (b) display mean ± SEM. The box and whisker plot in (c) and (e) shows medians, 25th/75th percentiles, and minimum and maximum values. Statistical significance for (a) and (b) was calculated using an ordinary, one-way ANOVA. Statistical significance for (c) and (e) was calculated using a Kruskal Wallis H test. Statistical significance for (f) was calculated using an unpaired, two-tail Student’s t-test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, n.s., non-significant.