Table 1. Macromolecule-production information.

The introduction of additional residues, the expression and purification tags, and TEV recognition sites are underlined. Cleavage sites are indicated with a slash.

	FANCM–CENP-SX	CENP-SX
Source organism	Gallus gallus	Gallus gallus
DNA source	cDNA	cDNA
Cloning vector	pMal-c2X	pRSFDuet-1
Expression vector	pMal-c2X	pRSFDuet-1
Expression host	E. coli One Shot BL21(DE3) pLysS RARE2	E. coli One Shot BL21(DE3) pLysS RARE2
Complete amino-acid sequence of the construct produced
CENP-S	MGSHHHHHHENLYFQ/GSEAAGGEQRELLIQRLRAAVHYTTGALAAQDVAEDKGVLFSKQTVAAISEITFRQAENFARDLEMFARHAKRSTITSEDVKLLARRSNSLLKYITQKSDELASSNMEQKEKKKKKSSAAKGRKTEENETPVTESEDSNMAT	MGSHHHHHHENLYFQ/GSEAAGGEQRELLIQRLRAAVHYTTGALAAQDVAEDKGVLFSKQTVAAISEITFRQAENFARDLEMFARHAKRSTITSEDVKLLARRSNSLLKYITQKSDELASSNMEQKEKKKKKSSAAKGRKTEENETPVTESEDSNMAT
CENP-X	MYWSHPQFEKENLYFQ/GYEEREGGFRKETVERLLRLHFRDGRTRVNGDALLLMAELLKVFVREAAARAARQAQAEDLEKVDIEHVEKVLPQLLLDFV	MYWSHPQFEKENLYFQ/GYEEREGGFRKETVERLLRLHFRDGRTRVNGDALLLMAELLKVFVREAAARAARQAQAEDLEKVDIEHVEKVLPQLLLDFV
FANCM	MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGIEGRHHHHHHENLYFQ/GRENLYFQ/GENLYFQ/GRSLHHKSALFSCVTDPKEMHCHENWSLSPEEFEIWDRLYRLKENDGVKEPILPHTRFETLENLDKTSKPEEEAAHKLSLSEWSIWQSRPFPTSMVDHSDRCYHFISVMELIEVMRQEQGDCSYELELQPHLRIEDIHVRRNKGHLSP