| Method |
Vapor diffusion, sitting drop |
Vapor diffusion, sitting drop |
| Plate type |
CrystalQuick Greiner plate, 3-well, round |
CrystalQuick Greiner plate, 3-well, round |
| Temperature (K) |
293 |
293 |
| Protein concentration (µM) |
50 (FANCM–CENP-SX pentamer) |
150 (CENP-SX tetramer) |
| DNA concentration (µM) |
55 |
55 |
| Buffer composition of protein–DNA solution |
10 mM MOPS–NaOH pH 7.0, 110 mM NaCl, 0.5 mM DTT |
10 mM Tris–HCl pH 7.5, 110 mM NaCl |
| Composition of reservoir solution |
50 mM MOPS–NaOH pH 7.0, 31% 1,4-dioxane, 10 mM MgCl2, 110 mM NaCl |
20 mM MES–NaOH pH 6.5, 40% MPD, 100 mM NaCl |
| Volume of reservoir (µl) |
100 |
100 |
| Volume and ratio of drop |
2 µl (1:1) |
2 µl (1:1) |
| Composition of harvesting solution |
50 mM MOPS–NaOH pH 7.0, 40% MPD, 10 mM MgCl2, 110 mM NaCl |
— |
| Cryoprotectant |
50 mM MOPS–NaOH pH 7.0, 30% MPD, 10 mM MgCl2, 110 mM NaCl, 30% ethylene glycol |
20 mM MES–NaOH pH 6.5, 40% MPD, 100 mM NaCl, 20% ethylene glycol, 15% DMSO |
