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. 2022 Apr 20;9:844047. doi: 10.3389/fcvm.2022.844047

FIGURE 4.

FIGURE 4

NRF2 and HO-1 inhibited cell senescence and promoted tube formation in HUVECs. (A) Western blotting showing the expression of HO-1 after overexpressing miR-181b-5p. **P < 0.01 vs. mimics NC. CCK-8 assay (B), β-gal staining and tube formation (C) showing the effects of siR-NRF2 or siR-HO-1 on cell viability, cell senescence and tube formation in HUVECs. *P < 0.05, **P < 0.01 vs. siR-NC. (D) Western blotting showing the PCNA, VEGF and p16INK4A expression in rescue experiments. *P < 0.05, **P < 0.01 vs. siR-NC. (E) CCK-8 assay showing cell viability in rescue experiments. **P < 0.01 vs. NC, #P < 0.05, ##P < 0.01 vs. miR-181b-5p inhibitor. (F) β-gal staining and tube formation showing cell senescence and tube formation ability in rescue experiments.