Figure 3.

(a) Volcano plot representations of significance and differential methylation in analysed fragments. Dotted lines represent thresholds of significance: −10LogP20, corresponding to P0.01 and mean methylation difference 10% (a) 4 ng/L vs control; B:120 ng/L vs control; C: 4 ng/L vs 120 ng/L. (b) Overall distribution of DMFs in genomic elements; (c) Venn Diagram showing specific and common DMFs between group comparisons; (d) Methylation levels (%) of common fragments between 4 vs Ctl and 120 vs Ctl comparisons (Da,Db), between 4 vs Ctl and 4 vs 120 comparisons (c–e) and between 120 vs Ctl and 4 vs 120 ng/L comparisons (f). For clearer representation of methylation differences, y axes start at 40%.