Figure 3.

Long-Term prophylactic protective efficacies of CFA+GLA-SE and CFA+GLA-SE/CDG immunization in a murine model of chronic progressive Mav-PI at 10 weeks post-infection. At 10 weeks post-infection, after euthanization of each group of immunized and infected mice (n = 6), (a) gross images and (b) 10× and 200× magnification photomicrographs with H&E staining (scale bars = 3 mm and 200 µm) of the right superior lobe of infected lung tissues were obtained; those of the entire groups are displayed. Each immunized group is indicated on the right side of the images in (a) and (b). (c) Quantitative analysis of the inflamed areas in the H&E-stained lung tissues. the sizes and percentages of the lesions in (b) are presented as a scatter plot with bars. The bacterial burdens (d) in the left lung lobe and half of the spleen in the mice from each group were assessed by counting viable bacterial colonies grown on 7H10-OADC agar plates, and the data are shown as in scatter plot with bars. Statistically significant differences among all groups in (c) and (d) were calculated by the unpaired t test, and the results are represented as the mean values along with the S.Ds. *p <.05, **p <.01 and ****p <.0001. The representative results are shown from a single in vivo experiment. CFA, culture filtrate antigen; Control, GLA-SE immunization alone; GLA-SE, glucopyranosyl lipid a adjuvant formulated in a stable oil-in-water emulsion; GLA-SE/CDG, GLA-SE plus cyclic-di-GMP.