TABLE 5.

Effects^a of calcium, heparin, and BSA on the inhibition of MTT reduction by ¹N^α-acylated analogues of CA(1-7)M(2-9) on L. donovani promastigotes

Assayb	% Inhibition
	CA(1-7)M(2-9) (1 μM)	Octα1-CA(1-7)M(2-9) (0.7 μM)	Lauα1-CA(1-7)M(2-9) (0.7 μM)
Standard conditions	82.1 ± 1.5	70.1 ± 7.5	78.9 ± 3.7
With Ca2+ at:
0.5 mM	60.6 ± 6.7	59.1 ± 3.5	58.2 ± 9.0
1.0 mM	41.2 ± 8.8	57.8 ± 9.3	60.4 ± 9.4
5.0 mM	17.2 ± 3.4	43.5 ± 9.8	52.4 ± 6.2
With heparin at:
0.01 mg/ml	5.4 ± 3.6	25.5 ± 6.9	53.5 ± 2.1
0.1 mg/ml	6.4 ± 5.3	26.5 ± 6.1	42.5 ± 3.7
1.0 mg/ml	3.3 ± 4.9	6.1 ± 1.3	31.4 ± 5.3
With BSA at:
0.1 mg/ml	78.5 ± 5.0	56.3 ± 4.3	46.5 ± 2.5
1.0 mg/ml	52.4 ± 1.9	32.7 ± 7.4	15.4 ± 3.9
10 mg/ml	17.9 ± 2.6	10.5 ± 1.7	4.1 ± 2.1

^aExpressed as percent inhibition of MTT reduction compared to the respective control in the absence of peptides. Values are means ± standard deviations. 

^bThe peptide and the corresponding agent were preincubated together for 15 min before being added to the parasite suspension.