Figure 1.

CMT-93 cells express protease-activated receptor 2 (PAR2). A: RT-PCR for PAR2 mRNA expression in CMT-93 cells. Reactions lacking reverse transcriptase enzyme (RT) and template control lacking cDNA were performed as controls. DNA ladder and the sizes (bp) are shown on the right of image, with expected product size being 167 bp (n = 3). B: Confocal immunocytochemistry for PAR2 in CMT-93 cell monolayers (n = 2). Green shows positive immunoreactivity for PAR2 and blue shows DAPI staining for nuclei. C: z stacks of optical sections through cell monolayers. Scale bar represents 10 μm.