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. 2022 May 1;19(1):609–621. doi: 10.1080/15476286.2022.2065116

Table 1.

Primer sequences

(A) Primers used to amplify genomic DNA of WT and ZNF598KO cells
Primer name
Sequence
Sde 57 (Fwd) 5’- GCTGTGCTGCGGAGACCTG −3’
Sde 59 (Rev)
5’- CAGGCCTCTCTCGGAAGCCG −3’
(B) Transcript isoform-specific primers used for qRT-PCR
Transcript
Primer name and sequence
ZNF598 Fwd (Sde34): 5’- AACCTCGACAAATGGTCCTG −3’
Rev (Sde35): 5’- GTCTTCGTCCTTGAGCTTCG −3’
Hnrnpl_NMD-insensitive Fwd (LC50): 5’- CAATCTCAGTGGACAAGGTG −3’
Rev (LC51): 5’- CTCCATATTCTGCGGGGTGA −3’
Hnrnpl_NMD-sensitive Fwd (LC47): 5’- GGTCGCAGTGTATGTTTGATG −3’
Rev (LC52): 5’- GGCGTTTGTTGGGGTTGCT −3’
TRA2B_NMD-insensitive Fwd (LC54): 5’- GAGGTTGGCAGCTTCGATT −3’
Rev (LC42): 5’- AAGCAGAACGGGATTCCC −3’
TRA2B_NMD-sensitive Fwd (LC43): 5’- TGGAATCAGAAAGCACTACGC −3’
Rev (LC55): 5’- GAATCTTCCTTGGAGCGAGA −3’
BAG1_NMD-insensitive Fwd (EK141): 5’- ACTCATATTTAAGGGAAAATCTCTG −3’
Rev (EK38): 5’- TTGGGCAGAAAACCCTGCTG −3’
BAG1_NMD-sensitive Fwd (EK139): 5’- CATATTTAAGGTTCTTCAACAGATA −3’
Rev (EK140): 5’- TGTTTCCATTTCCTTCAGAGA −3’
GAS5 Fwd (Schwi292): 5’- GCACCTTATGGACAGTTG −3’
Rev (Schwi293): 5’- GGAGCAGAACCATTAAGC −3’
RP9P Fwd (OM368): 5’- CAAGCGCCTGGAGTCCTTAA −3’
Rev (OM369): 5’- AGGAGGTTTTTCATAACTCGTGATCT −3’
SMG5 Fwd (SRE237): 5’- CCAGTGGCCGCTTCATTGTC −3’
Rev (SRE238): 5’- TGCCTCCAGGTACCGAATCC- 3’
Actin β Fwd (SRE61): 5’- TCCATCATGAAGTGTGACGT −3’
Rev (SRE62): 5’- TACTCCTGCTTGCTGATCCAC −3’