Table 1.
(A) Primers used to amplify genomic DNA of WT and ZNF598KO cells | ||
Primer name |
Sequence |
|
Sde 57 (Fwd) | 5’- GCTGTGCTGCGGAGACCTG −3’ | |
Sde 59 (Rev) |
5’- CAGGCCTCTCTCGGAAGCCG −3’ |
|
(B) Transcript isoform-specific primers used for qRT-PCR | ||
Transcript |
Primer name and sequence |
|
ZNF598 | Fwd (Sde34): 5’- AACCTCGACAAATGGTCCTG −3’ Rev (Sde35): 5’- GTCTTCGTCCTTGAGCTTCG −3’ |
|
Hnrnpl_NMD-insensitive | Fwd (LC50): 5’- CAATCTCAGTGGACAAGGTG −3’ Rev (LC51): 5’- CTCCATATTCTGCGGGGTGA −3’ |
|
Hnrnpl_NMD-sensitive | Fwd (LC47): 5’- GGTCGCAGTGTATGTTTGATG −3’ Rev (LC52): 5’- GGCGTTTGTTGGGGTTGCT −3’ |
|
TRA2B_NMD-insensitive | Fwd (LC54): 5’- GAGGTTGGCAGCTTCGATT −3’ Rev (LC42): 5’- AAGCAGAACGGGATTCCC −3’ |
|
TRA2B_NMD-sensitive | Fwd (LC43): 5’- TGGAATCAGAAAGCACTACGC −3’ Rev (LC55): 5’- GAATCTTCCTTGGAGCGAGA −3’ |
|
BAG1_NMD-insensitive | Fwd (EK141): 5’- ACTCATATTTAAGGGAAAATCTCTG −3’ Rev (EK38): 5’- TTGGGCAGAAAACCCTGCTG −3’ |
|
BAG1_NMD-sensitive | Fwd (EK139): 5’- CATATTTAAGGTTCTTCAACAGATA −3’ Rev (EK140): 5’- TGTTTCCATTTCCTTCAGAGA −3’ |
|
GAS5 | Fwd (Schwi292): 5’- GCACCTTATGGACAGTTG −3’ Rev (Schwi293): 5’- GGAGCAGAACCATTAAGC −3’ |
|
RP9P | Fwd (OM368): 5’- CAAGCGCCTGGAGTCCTTAA −3’ Rev (OM369): 5’- AGGAGGTTTTTCATAACTCGTGATCT −3’ |
|
SMG5 | Fwd (SRE237): 5’- CCAGTGGCCGCTTCATTGTC −3’ Rev (SRE238): 5’- TGCCTCCAGGTACCGAATCC- 3’ |
|
Actin β | Fwd (SRE61): 5’- TCCATCATGAAGTGTGACGT −3’ Rev (SRE62): 5’- TACTCCTGCTTGCTGATCCAC −3’ |