Figure 5.
pS273R inhibited cGAS-STING signaling mediated anti-VSV function. (a) PAMs in 24-well plates (3 × 105 cells/well) were co-transfected with 1 μg 3×FLAG-pCMV-S273R or empty vector for 24 h and then infected with .001 MOI or .01 MOI VSV for 16 h. the GFP signals were observed by fluorescence microscopy. (b) PAM cells in 6-well plates (8 × 105 cells/well) were co-transfected with 2 μg 3×FLAG-pCMV-S273R or control vector for 24 h and then infected with .001 MOI or .01 MOI VSV for 16 h, the GFP cells in infected PAMs were analyzed by flow cytometry. (c–g) the infected cells were harvested to measured VSV glycoprotein gene (c) and cellular gene transcriptions (e,f) by RT-qPCR. the viral titer in the supernatant from VSV infected PAMs was measured by TCID50 assay (d) and plaque assay (g).